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核桃树皮是一种常见的中草药,含有甙类及大量(?)质等。本文通过体外试验:台盼兰拒染法和~3H—TdR掺入法,检测了核桃树皮提取液对QGY人肝癌细胞的活性抑制作用。发现核桃树皮提取液对QGY细胞有毒性作用,而且实验组随着提取液浓度增加,其对QGY细胞的存活率影响呈剂量依赖性降低。当浓度为1:100时,QGY细胞存活率最低,与阳性对照组相近,两组无统计学差别。说明核桃树皮提取液能够杀伤体外培养的QGY细胞。此结果将对核桃树皮的开发利用提供试验依据。
Walnut bark is a common Chinese herbal medicine, containing apes and a large number of (?) quality. In this paper, the inhibitory effect of walnut bark extract on QGY human hepatocarcinoma cells was tested by in vitro tests: trypan blue exclusion and ~3H-TdR incorporation. Walnut bark extract was found to have toxic effects on QGY cells, and the experimental group showed a dose-dependent decrease in the survival rate of QGY cells as the extract concentration increased. When the concentration was 1:100, the survival rate of QGY cells was the lowest, which was similar to that of the positive control group. There was no statistical difference between the two groups. The results showed that walnut bark extract was able to kill QGY cells in vitro. This result will provide experimental basis for the development and utilization of walnut bark.