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目的:研究3种固定修复用粘结剂对人中性粒细胞(polymorph nuclear neutrophils,PMNs)相关活性以及致炎性等方面的影响。方法:在24孔板底部铺上玻璃离子水门汀、聚羧酸锌水门汀、光固化树脂水门汀,采集健康成人志愿者静脉血分离提纯PMNs,将PMNs与3种粘结剂接触培养4 h后,检测细胞的早期凋亡率、坏死率以及存活率的变化,产生的活性氧簇(reactiveoxygen species,ROS)以及分泌白介素(IL)-8的量。结果:3组粘结剂的早期凋亡率、坏死率和存活率两两比较,差异有统计学意义(P<0.05),其中聚羧酸锌水门汀组早期凋亡率、坏死率最高,存活率最低;玻璃离子水门汀组早期凋亡率、坏死率最低,存活率最高。玻璃离子水门汀组的早期凋亡率与存活率与空白对照组比较差异无统计学意义(P>0.05),而3组粘结剂的坏死率均高于空白对照组(P<0.05)。PMNs释放IL-8的量,3组粘结剂均高于空白对照组,其中聚羧酸锌水门汀组最高,玻璃离子水门汀组最低,差异有统计学意义(P<0.05)。PMNs的ROS产生强度,只有玻璃离子水门汀组高于空白对照组,差异有统计学意义(P<0.05)。结论:3种粘结剂对人中性粒细胞的相关活性以及致炎性均有影响,其中玻璃离子水门汀的致炎性相对较低,抗菌性能相对较高。
OBJECTIVE: To study the effects of three adhesives for fixation repair on the activity and inflammation of polymorphonuclear neutrophils (PMNs). Methods: Glass ionomer cement, polycarboxylate cement and light-cured resin cement were applied on the bottom of 24-well plate, and PMNs were isolated from venous blood of healthy adult volunteers. PMNs were incubated with three kinds of binders for 4 h, Changes in early apoptotic rate, necrosis rate and survival rate of cells, production of reactive oxygen species (ROS) and secretion of interleukin (IL) -8. Results: The early apoptotic rate, necrosis rate and survival rate of three groups of adhesive were compared with each other statistically, the difference was statistically significant (P <0.05), among them, the rate of early apoptosis and necrosis in polycarboxylate cement group was the highest, The lowest rate; glass ionomer cement group early apoptosis rate, the lowest necrosis, the highest survival rate. There was no significant difference in the early apoptosis rate and survival rate between the glass ionomer cement group and the blank control group (P> 0.05), but the necrosis rate of the three groups of cement injection group was higher than that of the blank control group (P <0.05). The amount of IL-8 released by PMNs was higher in the three groups than in the blank control group, of which the highest was in the group of polycarboxylate cement and the lowest was in the glass ionomer cement group (P <0.05). The production intensity of ROS of PMNs was higher than that of the blank control group, the difference was statistically significant (P <0.05). CONCLUSION: The three binders have effects on the activity of neutrophil and on the inflammatory activity. Glass ionomer cement has relatively low inflammatory activity and relatively high antibacterial activity.