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目的:研究FOXP3在食管癌中表达规律。方法:选择2010年1月~2010年10月在我院接受手术治疗的食管鳞癌患者48例,选取所有患者肿瘤组织及正常食管黏膜组织进行免疫组化分析,观察FOXP3在食管癌细胞系Eca-109、正常食管黏膜细胞系Eca-109、人食管癌组织、正常食管黏膜组织的表达,FOXP3在不同年龄、性别、肿瘤分化程度、肿瘤直径、淋巴结转移上的表达。结果:FOXP3在食管癌组织中阳性表达率(85.42%)显著高于正常食管黏膜组织(14.58%),P<0.05。FOXP3在正常细胞系表达为阴性,在食管癌细胞系Eca-109中的表达阳性,FOXP3在正常食管黏膜组织中表达阴性,在食管癌组织中表达阳性。在食管癌组织中,FOXP3阳性表达率和食管癌患者的性别、年龄等不同临床病理特征均无关联(P>0.05),和T分期、TNM分期、肿瘤直径、淋巴结转移、远处转移等存在关联(P<0.05)。结论:食管癌细胞能够表达FOXP3,且其表达水平和食管癌的进展关系密切,可以作为临床诊断及治疗食管癌的重要参考指标。
Objective: To study the expression of FOXP3 in esophageal cancer. Methods: Forty-eight patients with esophageal squamous cell carcinoma who underwent surgery in our hospital from January 2010 to October 2010 were enrolled in this study. Tumor tissues and normal esophageal mucosa tissues of all patients were selected for immunohistochemical analysis. FOXP3 expression in esophageal cancer cell lines Eca -109, normal esophageal mucosa cell line Eca-109, human esophageal cancer and normal esophageal mucosa, expression of FOXP3 in different age, gender, tumor differentiation, tumor diameter and lymph node metastasis. Results: The positive expression rate of FOXP3 in esophageal carcinoma was significantly higher than that in normal esophageal mucosa (85.42% vs 14.58%, P <0.05). FOXP3 is negative in normal cell lines and positive in esophageal cancer cell line Eca-109. FOXP3 is negative in normal esophageal mucosa tissues and positive in esophageal cancer tissues. In esophageal cancer, the positive rate of FOXP3 was not correlated with the clinicopathological characteristics of esophageal cancer patients (P> 0.05), such as T stage, TNM stage, tumor diameter, lymph node metastasis and distant metastasis (P <0.05). CONCLUSION: FOXP3 can be expressed in esophageal cancer cells, and its expression level is closely related to the progress of esophageal cancer, which may be used as an important reference for clinical diagnosis and treatment of esophageal cancer.