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本实验应用免疫组化－链霉素抗生物素蛋白－过氧化酶连接法（ＳＰ）对正常口腔粘膜、白斑和鳞癌中Ｐ５３、Ｃ－ｍｙｃ基因的表达进行研究．结果表明：正常粘膜无一例阳性；轻、中、重度异常增生白斑和鳞癌Ｐ５３蛋白表达率为１２．５％，２５．０％，３３．３％和５０．０％；Ｃ－ｍｙｃ表达率为０．０％，１２．５％，１６．７％和２３．３％．白斑和鳞癌间Ｐ５３变化有显著性差异，Ｃ－ｍｙｃ表达无显著性差异．此两基因的表达与鳞癌有否淋巴结转移无关，且在鳞癌发生中有协同作用
In this study, the expression of P53 and C-myc in normal oral mucosa, leukoplakia and squamous cell carcinoma was studied by immunohistochemistry-streptavidin-peroxidase (SP) method. The results showed that: none of the normal mucosa was positive; the expression rates of P53 protein were 12.5%, 25.0%, 33.3% and 50.0% in the patients with mild, moderate and severe dysplasia of leukoplakia and squamous cell carcinoma; the expression rate of C-myc 0.0%, 12.5%, 16.7% and 23.3%. There was a significant difference in P53 between leukoplakia and squamous cell carcinoma, but there was no significant difference in C-myc expression. The expression of these two genes has no relation with lymph node metastasis of squamous cell carcinoma and has a synergistic effect in the occurrence of squamous cell carcinoma