论文部分内容阅读
附睾是精子成熟的场所,附睾分泌的酶、激素和营养物质对精子成熟起着重要作用,其中蛋白水解酶介导的表面分子修饰或丢失是精子成熟的一个重要方面.纤溶酶(Plas.min)是一种丝氨酸蛋白水解酶,具广泛的蛋白水解酶活性,在细胞外蛋白水解中有重要作用纤溶酶原激活因子(PA)特异激活纤溶酶原转化为有活性的纤溶酶.PA抑制因子(PAI-1)能够特异中和PA活性.为研究附睾蛋白水解酶活性调节机制,本文对PA及PAI-1在大鼠附睾中的表达作了初步研究.结果表明:(1)附睾组织分级组织型(tPA)和尿激酶型(uPA)PA,二者活性受激素调节;(2)mRNA定位证实附睾上皮表达tPA和PAI-1.上述结果提示附睾有完善的PA调节系统,PA和PAI-1的协同表达在附睾蛋白水解酶活性调节中起重要作用.
Epididymis is the site of sperm maturation. Enzymes, hormones and nutrients secreted by the epididymis play an important role in sperm maturation. Proteolytic enzyme-mediated surface molecule modification or loss is an important aspect of sperm maturation. min) is a serine protease with extensive proteolytic activity that plays an important role in extracellular proteolysis Plasminogen activator (PA) -specific activation of plasminogen converts to active plasmin PA inhibitor (PAI-1) can specifically neutralize PA activity.In order to study the mechanism of the regulation of epididymal protease activity, PA and PAI-1 were preliminarily studied in epididymis of rats.The results showed that: (1 ) Epididymal grading tissue type (tPA) and urokinase type (uPA) PA, both of which are regulated by hormones; (2) mRNA localization confirms the expression of tPA and PAI-1 in epididymal epithelium.The above results suggest that epididymis has a perfect PA regulatory system The co-expression of PA and PAI-1 plays an important role in the regulation of epididymal protease activity.