日本甲型肝炎(HA)疫苗的研究始于1981年,当时厚生省举办了HA疫苗开发研究班。此后正式进行了HA疫苗开发研究。预防卫生研究所森次等采用非洲绿猴肾(GMK)细胞进行病毒培养繁殖,经分离、克隆(GL-37细胞),得到高度减毒的病毒株(KRM003)。经狨猴接种实验证明安全有效,但通过人体是否会恢复毒力,尚待确定。 嗣后,化学及血清疗法研究所等又研制了临床试用的细胞培养HA疫苗(DCK Research on the Japanese Hepatitis A (HA) vaccine began in 1981 when the Ministry of Health, Labor and Welfare hosted the HA Vaccine Development Seminar. Since then officially developed HA vaccine research. The Institute of Preventive Health Morimoto et al. Used GMK cells to culture and propagate the virus. The highly attenuated strain (KRM003) was isolated and cloned (GL-37 cells). The macaque vaccination experiment proved safe and effective, but whether the body will restore virulence remains to be determined. Later, the Institute of Chemistry and Serum Therapy and other developed a pilot trial of cell culture HA vaccine (DCK