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[Objective] To screen optimum conditions of assay method and thin-layer chromatography was used for qualitative discrimination of astragaloside Ⅳ contained in Jin Weng Zhili particle,which provide basis for quality standard. [Methods] According to method described in Chinese Veterinary Pharmacopoeia,thin-layer chromatography was used for qualitative discrimination of astragaloside Ⅳ contained in Jin Weng Zhili particle by improving extraction method and adjusting the proportion of ingredients in developing solvent. [Results] For identification of astragaloside Ⅳ in Jin Weng Zhili particle using thin-layer chromatography,the results were the optimum after chromatographic condition was improved,ie,extraction,column transferring,developing solvent of trichloromethane-methyl alcohol-water( 10 ∶ 5 ∶ 1) which is better than method described in Chinese Veterinary Pharmacopoeia. [Conclusions] The method is of good feasibility,repeatability and specificity which is benefit to the identification of astragaloside Ⅳ contained in Jin Weng Zhili particle using thin-layer chromatography.
[Objective] To screen optimum conditions of assay method and thin-layer chromatography was used for qualitative discrimination of astragaloside IV contained in Jin Weng Zhili particle, which provide basis for quality standard. [Methods] According to method described in Chinese Veterinary Pharmacopoeia, thin -layer chromatography was used for qualitative discrimination of astragaloside IV contained in Jin Weng Zhili particle by improving extraction method and adjusting the proportion of ingredients in developing solvent. [Results] For identification of astragaloside IV in Jin Weng Zhili particle using thin-layer chromatography, The results were the optimum after chromatographic condition was improved, ie, extraction, column transfer, developing solvent of trichloromethane-methyl alcohol-water (10: 5: 1) which is better than method described in Chinese Veterinary Pharmacopoeia. [Conclusions] The method is of good feasibility, repeatability and specificity which is benefit to the iden tification of astragaloside IV contained in Jin Weng Zhili particle using thin-layer chromatography.