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目的:建立测定通便胶囊中二苯乙烯苷含量的反相高效液相色谱法。方法:采用ZORBAX ODS C_(18)色谱柱(250mmmx4.6mm,5μm),流动相:乙腈-水(25:75);流速:1.0ml·min~(-1);检测波长:320nm;柱温:30℃。结果:二苯乙烯苷在1~250μg·ml~(-1)线性关系良好(r=0.9999);平均回收率为98.9%,RSD1.7%(n=6)。结论:该方法操作简便、快速,适用于通便胶囊中二苯乙烯苷的含量测定。
Objective: To establish a reversed-phase high performance liquid chromatography method for the determination of stilbene glycoside in Tongbian capsules. METHODS: A ZORBAX ODS C 18 column (250 mm x 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-water (25:75). The flow rate was 1.0 ml·min -1. The detection wavelength was 320 nm. : 30°C. RESULTS: The linearity of stilbene glycosides was between 1 and 250 μg·ml -1 (r=0.9999). The average recovery was 98.9% and RSD 1.7% (n=6). Conclusion: This method is simple, rapid and suitable for the determination of stilbene glycosides in Tongbian capsules.