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目的检测及探讨Ki67和Caspase-3在外阴癌变中的作用。方法选取1996年1月至2006年8月在中国医科大学附属第一医院外阴鳞癌(VSCC)30例,并收集其临床病理特征;外阴上皮内瘤变(VIN)30例;外阴上皮内非瘤样病变(NNED)30例;正常外阴皮肤10例。采用鼠抗人Ki67单克隆抗体及兔抗人Caspase-3多克隆抗体以SP免疫组化法进行研究。结果Ki67在外阴鳞癌中高表达(阳性率83·3%),在VIN及NNED中表达下降,在外阴正常皮肤中低表达。Caspase-3在外阴鳞癌中低表达(阳性率53·3%),在VIN及NNED及外阴正常皮肤中表达增加。Caspase-3的表达随外阴鳞癌的分化程度降低而降低(P<0·05)。Caspase-3蛋白和Ki67蛋白在30例外阴鳞癌中的表达呈负相关(P<0·05)。结论细胞增殖标记抗原Ki67的表达阳性率增高与外阴癌的发生密切相关。细胞凋亡相关蛋白Caspase-3参与了外阴癌的发生过程,并且可能作为评价外阴癌侵袭性的一个指标。
Objective To detect and explore the role of Ki67 and Caspase-3 in vulvar carcinogenesis. Methods Thirty patients with vulvar squamous cell carcinoma (VSCC) at the First Affiliated Hospital of China Medical University from January 1996 to August 2006 were selected and their clinical and pathological features were collected. 30 cases of vulvar intraepithelial neoplasia (VIN) Tumor-like lesions (NNED) in 30 cases; normal vulva skin in 10 cases. Mouse anti-human Ki67 monoclonal antibody and rabbit anti-human Caspase-3 polyclonal antibody were used SP immunohistochemistry. Results Ki67 was highly expressed in vulvar squamous cell carcinoma (positive rate 83.3%), down-regulated in VIN and NNED, and low in normal skin of vulva. Caspase-3 expression in vulvar squamous cell carcinoma (53.3% positive), in VIN and NNED and vulvar normal skin expression increased. The expression of Caspase-3 decreased with the differentiation of vulvar squamous cell carcinoma (P <0.05). Caspase-3 protein and Ki67 protein expression in 30 cases of vulvar squamous cell carcinoma was negatively correlated (P <0.05). Conclusion The positive rate of Ki67 in cell proliferation is closely related to the occurrence of vulvar cancer. Caspase-3, an apoptosis-related protein, is involved in the pathogenesis of vulvar cancer and may be used as an indicator to evaluate the aggressiveness of vulvar cancer.