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植物名称:刺梨(Rosa roxburghii)。材料类别:由贵州引种栽培的三年生植株的茎梢和带腋芽的嫩茎,消毒后切成1—1.5cm长的切段。培养条件:以MS为基本培养基。诱导芽增殖培养基:(1)每升附加6-BA0.5mg;(2)每升附加6-BA0.5mg,NAA0.01—0.05mg;蔗糖3—5％,琼脂0.7％。诱导生根培养基:每升附加 NAA0.2—1.0mg,蔗糖2—3％。培养室温度25±1℃,每天用日光灯辅助照明10—11小时,光照度1000—2000lx。生长与分化情况:将茎段按植株的生长极性插
Plant name: Rosa roxburghii. Material Category: Three-year-old plant stems and shoots with axillary buds introduced and cultivated in Guizhou were cut into 1-1.5 cm long sections after disinfection. Culture conditions: MS as a basic medium. Induction of bud proliferation medium: (1) Additional 6-BA 0.5 mg per liter; (2) Additional 6-BA 0.5 mg, NAA 0.01-0.05 mg per liter; 3-5% sucrose, 0.7% agar. Induction rooting medium: NAA 0.2-1.0mg / liter, 2-3% sucrose. Incubation room temperature 25 ± 1 ℃, daily lighting with fluorescent lighting 10-11 hours, illumination 1000-2000lx. Growth and differentiation: the stem segments according to the growth of plants inserted