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用酚水法从 2 0dm3 大黄鱼的病原菌———溶藻弧菌菌液分离出脂多糖 (LPS)溶液380cm3 ,冻干后得到LPS粉末 97.6mg .在注射剂量为 0 .2cm3 不同浓度LPS溶液对大黄鱼肌肉注射毒性试验中 ,LPS注射浓度为 0 .5mg/cm3 时 ,5d内能引起大黄鱼死亡 ;当LPS浓度提高到 2mg/cm3 时 ,大黄鱼的死亡率达到 1 0 0 % .以剂量为 0 .2cm3 、浓度为 0 .2mg/cm3 的LPS溶液对大黄鱼进行免疫注射 ,1 5d后用 0 .2cm3 浓度为 9× 1 0 8个 /cm3 的溶藻弧菌进行攻毒试验 ,4 0 %的大黄鱼获得免疫保护
Phenol water method from 20dm3 large yellow croaker pathogenic bacteria --- Vibrio alginolyticus isolated lipopolysaccharide (LPS) solution 380cm3, lyophilized to give LPS powder 97.6mg in the injection dose of 0. 2cm3 different concentrations of LPS solution In the intramuscular injection toxicity test of large yellow croaker, the death of big yellow croaker could be caused within 5 days when LPS injection concentration was 0.5 mg / cm 3, and the mortality rate of large yellow croaker reached 100% when LPS concentration was increased to 2 mg / cm 3. Large yellow croaker was injected by LPS at a dose of 0.2cm3 and a concentration of 0.2mg / cm3, and challenged with V. alginolyticus at a concentration of 0.2cm3 at 9 × 108 / cm3 after 15 days. 40% of the big yellow croaker was immunized