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分析了采用耐热细菌LDH酶制备的生物传感器对汞和镍的响应性。将酶纯化、固定在一个涂有PGA-吡咯聚合材料的金箔上作为工作电极,在增加溶液中乳酸浓度的情况下测定生物传感器对两种不同浓度的汞和镍溶液的响应性。当溶液中含25×10-7mmol/L汞和17×10-5mmol/L镍时,生物传感器的电流响应分别从0.32μA下降到0.09μA和从4.13μA下降到2.63μA。在含25×10-7mmol/L汞溶液中,生物传感器的敏感性从0.0102μA/(mmol·L-1)下降到0.0043μA/(mmol·L-1)。另一方面,镍的存在并没有导致传感器敏感性的降低。结果表明,制备的生物传感器适合用于含汞和镍溶液中的汞检测。
The biosensors prepared with the thermophilic bacteria LDH enzyme were analyzed for mercury and nickel responsiveness. The enzyme was purified and immobilized on a gold foil coated with PGA-pyrrole polymer as a working electrode. The responsiveness of the biosensor to two different concentrations of mercury and nickel solutions was determined while increasing the lactate concentration in solution. When the solution contained 25 × 10-7mmol / L mercury and 17 × 10-5mmol / L nickel, the biosensor’s current response decreased from 0.32μA to 0.09μA and from 4.13μA to 2.63μA, respectively. The biosensor sensitivity decreased from 0.0102μA / (mmol·L-1) to 0.0043μA / (mmol·L-1) in 25 × 10-7mmol / L mercury solution. On the other hand, the presence of nickel did not lead to a decrease in sensor sensitivity. The results show that the prepared biosensors are suitable for mercury detection in mercury and nickel solutions.