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利用溴化脱氧尿嘧啶（Ｂｒｄｕ）作为胸苷类似物可掺入到新合成的ＤＮＡ中的特点,通过检测Ｂｒｄｕ标记的细胞便能定性、定量地反映细胞的增殖状态〔１〕。我们已筛选出分泌特异性抗Ｂｒｄｕ单克隆抗体（ｍＡｂ）的杂交瘤细胞株〔２〕。为制备荧光素标记的抗ＢｒｄｕｍＡｂ用于
The use of bromodeoxyuridine (Brdu) as a thymidine analogues can be incorporated into the newly synthesized DNA characteristics, by detecting Brdu labeled cells can be qualitatively and quantitatively reflect the proliferation state of cells 〔1〕. We have screened hybridoma cell line secreting specific anti-Brdu monoclonal antibody (mAb) 〔2〕. Fluorescein-labeled anti-BrdumAb was used for the preparation