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将含有乙型肝炎表面抗原（ＨＢｓＡｇ）基因的重组质粒ｐＡｃｔ－ＭＳ直接注入小鼠骨骼肌中，２周后加强免疫１次。免疫后４～９周间，每周检测小鼠血清中ＨＢｓ。结果表明实验组６／８只小鼠ＨＢｓ为阳性，对照组８只小鼠均未检测到ＨＢｓ。本实验证实直接注射质粒ＤＮＡ外源基因能获得表达，并能引起免疫反应。
The recombinant plasmid pAct-MS containing hepatitis B surface antigen (HBsAg) gene was injected directly into mouse skeletal muscle and boosted once after 2 weeks. Between 4 and 9 weeks after immunization, the serum levels of HBs in mice were detected weekly. The results showed that 6/8 mice in the experimental group were positive for HBs and none of the 8 mice in the control group. This experiment confirmed that direct injection of plasmid DNA exogenous gene expression can be obtained and can cause immune response.