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目的探讨胃肠疾病患者口腔与胃黏膜寄居念珠菌菌株基因型的相关性。方法对154例因消化不良就诊的患者分别采集口腔及胃黏膜标本、分别用念珠菌显色培养基分离鉴定念珠菌,进行内转录间隔区(internal transcribed spacer region,ITS)基因测序并申请GenBank登录序列号,观察同一患者口腔和胃黏膜念珠菌菌种是否一致。在菌种一致患者中,随机抽取其口腔和胃黏膜分离菌株进行多位点序列分型(multilocus sequencing typing,MLST),并分析口腔及胃黏膜念珠菌基因型进化情况。结果 46例(占29.87%)患者口腔与胃黏膜同时检出念珠菌,其中,37例(占80.43%)患者口、胃分离株均为白色念珠菌:包括胃炎患者25例,溃疡患者11例,胃癌患者1例。随机选取来自24例患者的48个分离株进行白色念珠菌MLST分型,结果显示,20例(占83.33%)患者的双标本基因型一致。结论胃肠疾病患者口腔与胃黏膜念珠菌菌种和基因型一致率较高,且消化性溃疡患者感染率高于胃炎患者。
Objective To investigate the correlation between oral and gastric mucosal Candida albicans genotypes in patients with gastrointestinal diseases. Methods Oral and gastric mucosal specimens were collected from 154 patients with dyspepsia. Candida were isolated and identified by Candida chromogenic medium, and the ITS sequences were sequenced and submitted to GenBank for registration Serial number, observe the same patient oral and gastric mucosal Candida species are consistent. In patients with consistent bacterial species, oral and gastric mucosal isolates were randomly selected for multilocus sequencing typing (MLST) and analyzed for the evolution of oral and gastric mucosal Candida genotypes. Results Forty-six patients (29.87%) had Candida albicans detected in both oral cavity and gastric mucosa. Among them, 37 (80.43%) patients had oral and gastric isolates of Candida albicans including 25 cases of gastritis and 11 cases of ulcer 1 case of gastric cancer. Forty-eight isolates from 24 patients were randomly selected for MLST typing of Candida albicans. The results showed that 20 cases (83.33%) had identical genotypes. Conclusion The consistency of oral and gastric mucosal Candida species and genotypes in patients with gastrointestinal diseases is high, and the prevalence of peptic ulcer patients is higher than that of gastritis patients.