论文部分内容阅读
研究了大田期氮磷钾营养及配比对白肋烟中有关保护酶的影响及相关物质的动态。结果表明,过氧化物酶(POD)活性与脯氨酸(Pro)含量在烟株生长前期表现为上升趋势、后期呈现下降趋势,峰期出现在移栽后65d左右;多酚氧化酶(PPO)活性与总酚、类黄酮的含量变化呈现出随生育进程的推进而增加的单峰曲线,至移栽后75d达到高峰后回落;丙二醛(MDA)含量也随生育进程的推进而上升,总游离氨基酸(AA)含量则随生育进程的推进而逐渐下降。氮磷钾施用量与白肋烟内原保护酶活性及相关物质含量均呈正相关,N与总酚含量的相关性达极显著水平,N与POD、Pro、PPO和淀粉酶含量的相关性达显著水平。三元素对白肋烟中几种保护酶活性及相关物质含量的影响程度不完全相同,对PPO、Pro、总游离氨基酸和淀粉酶的影响程度为:N>P>K;对MDA、类黄酮含量的影响:K>N>P;对POD和总酚的影响:N>K>P。
The effects of N, P, K nutrition and proportioning on the protective enzymes in burley tobacco during the field and the dynamics of related substances were studied. The results showed that peroxidase (POD) activity and proline (Pro) content showed an upward trend in the early growth stage of tobacco plants, and showed a downward trend in the late stage, with a peak at about 65 days after transplanting. Polyphenol oxidase (PPO) ) Activity and the content of total phenols and flavonoids showed a unimodal curve increased with the progress of fertility, and then dropped after reaching the peak 75 days after transplanting. The content of malondialdehyde (MDA) also increased with the progress of the growth process , Total free amino acid (AA) content with the progress of the fertility process and gradually decreased. There was a positive correlation between the content of N, P and K and the activity of protective enzymes and related substances in burley tobacco, the correlation between N and total phenolic content was extremely significant, and the correlation between N and POD, Pro, PPO and amylase content was significant Level. The effects of three elements on the activities of several protective enzymes and related substances in burley tobacco were not completely the same, and the degrees of influence on PPO, Pro, total free amino acids and amylase were: N> P> K; Effect of K> N> P on POD and total phenol: N> K> P.