肝脏灌流技术在现代药理学研究中已广泛应用。利用灌流方法可以提供在体和离体肝脏所必需的营养成分和生理环境。与肝组织匀浆,肝组织切片,肝细胞分离,肝细胞膜,以及肝细胞酶提取等研究方法相比,肝脏灌流方法的特点是肝脏的组织结核和门脉系统,肝血窦及Disse间隙等参予物质转运的结构均不受影响。该技术可用于药物代谢,代谢动力学,药 Liver perfusion technique has been widely used in modern pharmacological studies. The perfusion method provides the nutrients and physiological environment necessary for in vivo and ex vivo liver. Compared with liver tissue homogenates, liver sections, liver cells, liver cell membrane, and liver cell enzyme extraction and other research methods, liver perfusion method is characterized by liver tissue and portal vein system, hepatic sinusoids and Disse gap The structure involved in material transport are not affected. The technology can be used for drug metabolism, metabolic dynamics, medicine