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目的:探讨同一个体食管贲门双源癌组织mdm2、Bcl-2、bax和p53蛋白变化特征及其意义。方法:采用免疫组化卵白素-生物素-过氧化物酶复合物(ABC)法和组织病理学方法,分析15例双源癌患者(同时发生食管鳞癌和贲门腺癌)mdm2、Bcl-2、bax和p53蛋白的表达状况。结果:15例食管和贲门双源癌患者食管鳞癌和贲门腺癌组织均出现不同程度的mdm2、Bcl-2、bax和p53蛋白的阳性表达,食管癌组织中免疫阳性率分别为67%(10/15)、87%(13/15)、73%(11/15)和73%(11/15);贲门癌组织中分别为67%(10/15)、80%(12/15)、80%(12/15)和67%(10/15),且免疫反应类型主要为弥漫型。mdm2、Bcl-2、bax和p53蛋白在食管和贲门双源癌肿瘤组织中一致性改变率分别为100%(15/15)、80%(12/15)、67%(10/15)和80%(12/15)。结论:食管和贲门双源癌存在较高的mdm2、Bcl-2、bax和p53蛋白表达一致性改变,提示食管贲门双源癌可能具有相似的发病因素和分子机制。
OBJECTIVE: To investigate the changes of mdm2, Bcl-2, bax and p53 protein in the same esophageal and gastric cardia carcinoma and its significance. Methods: Immunohistochemistry avidin-biotin-peroxidase complex (ABC) method and histopathological method were used to analyze the expression of mdm2, Bcl-2 and p53 in 15 patients with double-origin cancer (esophageal squamous cell carcinoma and gastric cardia adenocarcinoma) 2, bax and p53 protein expression status. Results: The positive expression of mdm2, Bcl-2, bax and p53 in esophageal squamous cell carcinoma and gastric cardia adenocarcinoma tissues were all found in 15 esophageal and gastric cardia cancer patients. The positive rate of esophageal cancer was 67% (10/15), 87% (13/15), 73% (11/15) and 73% (11/15) , 80% (12/15) and 67% (10/15) respectively, and the type of immune reaction was mainly diffuse. The consistent rates of change of mdm2, Bcl-2, bax and p53 protein in esophageal and gastric cardiac cancer were 100% (15/15), 80% (12/15), 67% (10/15) and 80% (12/15). CONCLUSION: The high expression of mdm2, Bcl-2, bax and p53 in esophageal and gastric cardial carcinomas has a consistent change, suggesting that esophageal and gastric cardial carcinoma may have similar pathogenesis and molecular mechanisms.