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1.植物名称枇杷(Eriobotrya japonica)。 2.材料类别当年生或一年生实生苗枝条的顶芽。取正在生长或芽已进入休眠(包括夏眠)的枝条的顶端1~1.5厘米,然后剥取2~5毫米长的茎尖。 3.培养条件基本培养基为MS培养基,促使茎尖转绿、展叶,需添加以下激素(毫克/升):BA0.5,NAA0.1,GA0.2;促其长成幼枝需添加BA0.25,IAA0.05;促使幼枝腋芽萌发增殖,则添加BA2。生根培养基为不加激素的1/2MS。试管苗经锻炼,先砂培然后土培。培养
1. Plant name loquat (Eriobotrya japonica). 2. Materials Category Annual shoots or annual shoots of young shoots. Take 1 to 1.5 cm from the top of shoots that are growing or the buds have entered hibernation, including the hibernation, and then petiole 2 to 5 mm long. 3. Culture conditions The basic medium for the MS medium, to promote the shoot tip turn green, Zhanye, need to add the following hormones (mg / l): BA0.5, NAA0.1, GA0.2; Add BA0.25, IAA0.05; Promote young shoots axillary buds germination and proliferation, then add BA2. Rooting medium without hormone 1 / 2MS. Test tube seedlings by training, then sand culture and then soil culture. to cultivate