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目的:研究转染p(21WAF1/CIP1)对肾癌的促凋亡作用.方法:采用脂质体介导的逆转录病毒载体将p21(WAF1/CIP1)转入无P21蛋白表达的肾癌细胞系GRC-1中,通过电镜、流氏细胞仪、DNA梯电泳检测细胞凋亡.结果:电镜检测到凋亡细胞,DNA电泳呈梯状.所检测细胞中15.3%出现凋亡.结论:p21(WAF1/CIP1)过表达有促进GRC-1细胞凋亡作用.
Objective: To investigate the effect of transfecting p (21WAF1 / CIP1) on the apoptosis of renal cell carcinoma. Methods: p21 (WAF1 / CIP1) was transfected into the cell line GRC-1 without P21 protein by liposome-mediated retroviral vector. Cell apoptosis was detected by electron microscopy, flow cytometry and DNA ladder electrophoresis Death. Results: Apoptotic cells were detected by electron microscopy and the DNA electrophoresis was ladder-shaped. 15.3% of the cells tested showed apoptosis. Conclusion: overexpression of p21 (WAF1 / CIP1) can promote the apoptosis of GRC-1 cells.