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目的探讨实时荧光聚合酶链反应(FQ-PCR)检测HBV-DNA与乙型肝炎病毒五项血清标志物(HBV-M)的关系。方法采用FQ-PCR技术和酶联免疫吸附法(ELISA)分别检测637例患者血清中的HBV-DNA拷贝数和乙肝五项血清标志物。结果HBV-DNA在“大三阳”组(HBsAg+HBeAg+抗HBc+和HBsAg+HBeAg+)和“小三阳”组(HBsAg+抗HBe+抗HBc+和HBsAg+抗HBc+)可检出,在“单纯抗体阳性”组(抗HBs+抗HBe+抗HBc+、抗HBs+抗HBc+、抗HBs+抗HBe+、抗HBs+、抗HBe+抗HBc+及抗HBc+)未检出。“大三阳”组患者血清中HBV-DNA检出率为92.13%,且82.68%的患者HBV-DNA拷贝数在105copies/ml以上;“小三阳”组患者血清中HBV-DNA检出率为44.39%,HBV-DNA拷贝数分布范围广。结论HBeAg的存在可间接反映HBV在体内的复制状态,而HBV-DNA的检测更客观地反映了HBV复制的程度和传染性。
Objective To investigate the relationship between HBV-DNA and five serological markers of hepatitis B virus (HBV-M) by real-time fluorescence polymerase chain reaction (FQ-PCR). Methods The serum HBV-DNA copy number and five serum hepatitis B markers of 637 patients were detected by FQ-PCR and ELISA respectively. Results HBV-DNA was detected in HBsAg + HBeAg + HBeAg + and HBsAg + HBeAg + and HBsAg + HBsAg + HBsAg + Antibody (anti-HBs + anti-HBe + anti-HBc +, anti-HBs + anti-HBc +, anti-HBs + anti-HBe +, anti-HBs +, anti- HBe + anti-HBc + and anti-HBc +) were not detected. The detection rate of HBV-DNA in serum of patients with “Sanyang” was 92.13%, and the copy number of HBV-DNA was above 105copies / ml in 82.68% of the patients; HBV-DNA The detection rate was 44.39%, HBV-DNA copy number distribution range. Conclusion The presence of HBeAg indirectly reflects the replication status of HBV in vivo, and the detection of HBV-DNA reflects the extent and infectivity of HBV replication more objectively.