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目的:观察益气补肾中药对自然流产模型小鼠脾脏、母胎界面组织NK细胞表达的影响。方法:以雌性CBA/J和雄性DBA/2为模型,孕0天始灌服益气补肾中药,并与环孢素A(CSA)对照,孕9天、14天流式分析脾脏、母胎界面NK细胞的表达。结果:孕9天各用药组NK细胞在脾脏及母胎界面的表达较阴性对照组明显降低(P<0.01或P<0.05);孕14天各用药组NK细胞在脾脏的表达较阴性对照组明显降低(P<0.05);孕14天中西医结合组NK细胞在母胎界面的表达较阴性对照组明显降低(P<0.05),而中药组、CSA组与阴性对照组比较无明显差异(P>0.05)。结论:益气补肾中药可下调自然流产模型小鼠孕9天及14天脾脏NK细胞表达及孕9天母胎界面NK细胞表达;益气补肾中药与CSA协同可下调自然流产模型小鼠孕14天母胎界面NK细胞表达,具有一定的免疫调节作用。
OBJECTIVE: To observe the effect of Yiqi Bushen Chinese herbs on NK cell expression in the spleen and maternal-fetal interface of spontaneous abortion model mice. Methods: The female CBA / J and male DBA / 2 models were fed with Yiqi Bushen Chinese medicine on the 0th day of pregnancy and compared with CsA, and the spleen and maternal fetal interface NK cell expression. Results: The expression of NK cells in the spleen and maternal-fetal interface at 9th day of gestation was significantly lower than that in the negative control group (P <0.01 or P <0.05) (P <0.05). Compared with the negative control group, the expression of NK cells in the Integrative Medicine group at the 14th day of gestation was significantly lower than that in the negative control group (P <0.05), while there was no significant difference between the Chinese medicine group and the CSA group and the negative control group (P> 0.05). Conclusion: Yiqi Bushen Chinese herbs can reduce spontaneous abortion mouse model of 9th and 14th days of spleen NK cells expression and 9 days of pregnancy fetal cell surface NK cell expression; Qi Qi and kidney combined with CSA can reduce the spontaneous abortion model mice 14 days pregnant Maternal-fetal interface NK cell expression, with a certain degree of immune regulation.