【摘 要】
：
Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of colchicine on HSCs growth were measured by MTT assay.
论文部分内容阅读
Cultured HSCs were treated colchicine with different concentrations for 12 h, respectively. The effects of colchicine on HSCs growth were measured by MTT assay. Effects of colchicine on gene expression of HSCs were analysed by using a self-made oligonucleotide microarray. Colchicine inhibited HSCs growth in a dose-dependent manner. After 12 h of treatment with 6.25 mg/L of colchicine, the expression of tissue inhibitor of metalloprotenase1 (TIMP-1) and the expression of interleukin-6 (IL-6) in HSCs were downregulated by 2.3 folds and 2.1 folds, respectively. These results suggest that colchicine's beneficial effects may, at least in part, owe to the inhibitory to the proliferation of HSCs and down-regulation of the expression of both TIMP1 and IL-6 in HSCs.
其他文献
目的:基于一般学习模型,验证暴力游戏对助人行为的消极作用,并探索暴力游戏在赋予亲社会背景后对助人行为的影响。方法:采用七巧板任务和情境模拟任务测量86名三年级儿童的稳
目的：考察亲社会行为类型（纯粹利他和互惠利他）在时间定价影响亲社会行为中的作用，为影响个体幸福感的政府企业决策提供参考建议。方法：采用2（实验操纵：时间定价组和控制组）×2（亲社会
GINS, a heterotetramer of SLD5, PSF1, PSF2, and PSF3 proteins, is an emerging chromatin factor recognized to be involved in the initiation and elongation step o
蛋白质是组成人体的主要固态成分,是生命活动的基础.近年来,由于蛋白质供给数量不足、质量低劣、资源严重浪费等问题得出现,人们更加注重对蛋白质的研究和开发,并通过利用传
丙酮酸及系列产品在化工、生化、制药和农用化学品等工业及科学研究中有着广泛的用途.丙酮酸及系列产品的纯度、含量、杂质等质量指标与合成方法有关,不同的应用途径对这些指
建立气相色谱法测定空气中丙二醇的含量.以乙醇为溶剂,富集空气中的丙二醇,再用DB-FFAP毛细管柱程序升温分离乙醇中的丙二醇,最后计算空气中丙二醇的含量.丙二醇在10.56～105.6
在波兰一项预防儿童铅中毒的项目中,研究者分析了11,877名年龄在24~84个月儿童的记录,评估了不同取样季节儿童的铅暴露与血铅水平的关系、问卷数据和环境铅水平。作为周围环
根据氨基酸分析仪的分离原理,分析缓冲液和显色剂国产化过程中常出现的问题,并且提出相应的解决方案,从而大大降低了氨基酸分析成本.
本实验对牛性别鉴定后IVP胚胎的冷冻方法及相关技术进行研究,目的是改善其冷冻效果,提高牛性别鉴定后IVP胚胎的移植妊娠率.实验结果如下:1、性别鉴定后IVP胚胎不能用常规冷冻
本研究中,利用差异显示法,在碳酸盐逆境下,从水稻(OryzasativaL.)根的cDNA文库中克隆得到了水稻热激蛋白90基因(rHsp90,GenBankAccessionNo.AB037681)。Northern杂交结果表明