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目的用常规细胞遗传学(conventional cytogenetics,CC)和荧光原位杂交(fluorescence chromosomal in situ hybridization,FISH)技术检测Ph染色体。方法常规细胞遗传学分析(CC),荧光原位杂交(FISH)技术。结果7例患者4例Ph染色体阴性,其中2例分别伴有t(18;22)和t(17;22)异常,其余2例为异基因造血干细胞移植后正常核型。一例培养后无中期分裂相。2例Ph染色体阳性,FISH结果bcr/abl(+)细胞检出率分别为63.87%,84.51%,7.56%,4.0%,74.45%,67%,47%。结论常规细胞遗传学与荧光原位杂交技术相结合对CML患者诊断治疗过程中肿瘤负荷动态检测有显著意义。
Objective To detect Ph chromosome by conventional cytogenetics (CC) and fluorescence chromosomal in situ hybridization (FISH). Methods Conventional cytogenetic analysis (CC) and fluorescence in situ hybridization (FISH) were performed. Results Four cases of Ph chromosome were negative in 7 patients. Two of them were associated with abnormalities of t (18; 22) and t (17; 22), respectively. The other two cases were normal karyotype after allogeneic hematopoietic stem cell transplantation. One case after culture without mitotic phase. Ph chromosome was positive in two cases. The positive rates of bcr / abl (+) cells in FISH were 63.87%, 84.51%, 7.56%, 4.0%, 74.45%, 67% and 47%, respectively. Conclusion The combination of routine cytogenetics and fluorescence in situ hybridization is of great significance in the dynamic detection of tumor burden in the diagnosis and treatment of CML patients.