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为了解杏自交不亲和性强度与授粉受精相关特性的关系,以自交亲和(Self-compatibility:SC)品种凯特(Prunus armeniaca L．cv．Katy)和自交不亲和(Self-incompatibility:SI)品种新世纪(P.armeniaca L.cv．Xinshiji)及凯特×新世纪杂种群体为试材,荧光显微镜观察自交亲和与自交不亲和杏花粉管生长动态。结果表明,授粉后初期,自交亲和性与自交不亲和性的杏花粉都能正常萌发、生长,但是在花粉管生长延伸到花柱1／2以后,自交亲和性的花粉管能顺利进入子房,而自交不亲和性的花粉管多数顶端膨大呈球形,停止向下生长,只有极个别能正常生长到达子房;杂种后代的可溶性蛋白含量和RNA酶比活力,与亲本相比无明显的趋中变异表现,而且在自交亲和与自交不亲和杏之间无显著性差异。
In order to understand the relationship between the selfing incompatibility of apricot and the related characteristics of pollination and fertilization, the self-compatibility (SC) variety Prunus armeniaca L.cv. Katy and self-incompatibility (Self- incompatibility (SI) cultivars (P.armeniaca L.cv.Xinshiji) and Kate × New Century hybrids were used as materials to observe the growth dynamics of self-compatible and self-incompatible apricot pollen tubes by fluorescence microscopy. The results showed that apricot pollen with self-compatibility and self-incompatibility could germinate and grow normally in the early stage after pollination. However, after the pollen tube grew to 1/2 of the style, self-compatible pollen tube Can successfully enter the ovary, and self-incompatibility of the majority of the top of the pollen tube enlarged spherical, stop downward growth, only very few can grow normally reach the ovary; hybrids of soluble protein content and RNase specific activity, and There was no significant trend of variation in inbred parents, but there was no significant difference between self-incompatibility and self-incompatibility apricot.