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目的:观察经前三剂止痛方(JQF)对小鼠免疫功能和应激反应的调节作用,通过现代医学的概念来初步研究方剂中所包含的扶正祛邪的中医内涵。方法:KM小鼠,雌雄各半,分为正常对照组、模型对照组、JQF高剂量组(1.9167 g·kg-1体重)、JQF低剂量组(0.9583 g·kg-1体重)和绞股蓝组(0.0237 g·kg-1体重),观察JQF对免疫功能低下小鼠的影响;将小鼠分为正常对照组、JQF高剂量组(1.9167 g·kg-1体重)、JQF低剂量组(0.9583 g·kg-1体重)和十全大补丸组(0.0156 g·kg-1体重)进行JQF抗应激实验:耐缺氧实验、耐疲劳实验、耐寒冷实验,分别计算小鼠在严重缺氧环境下的存活时间,在极度寒冷条件下的死亡百分率及小鼠因疲劳致死的负重游泳时间。结果:经前三剂止痛方对于免疫功能低下的小鼠以及在严重缺氧、寒冷、疲劳条件下小鼠的应激反应均有良好的修复作用。结论:本研究通过小鼠的免疫、应激实验显示,在扶正祛邪治则指导下拟定的活血养血方剂——经前三剂止痛方具有提高小鼠免疫功能和调节应激反应作用,对探讨祖国医学扶正祛邪治则客观化具有指导意义。
Objective: To observe the regulation effect of JQF on immune function and stress response in mice and to study the connotation of traditional Chinese medicine (TCM) contained in prescriptions through the concept of modern medicine. Methods: KM mice were divided into normal control group, model control group, JQF high dose group (1.9167 g · kg -1 body weight), JQF low dose group (0.9583 g · kg -1 body weight) and Gynostemma (0.0237 g · kg-1 body weight). The mice were divided into normal control group, JQF high dose group (1.9167 g · kg-1 body weight), JQF low dose group (0.9583 kg · kg-1 body weight) and Shiquan Dabu pill group (0.0156 g · kg-1 body weight) were subjected to JQF anti-stress test: hypoxia tolerance test, fatigue resistance test and cold tolerance test. Survival time in oxygen environment, percentage of death in extremely cold conditions and weight-bearing swimming time in mice due to fatigue. Results: The first three doses of analgesic prescription for immune dysfunction in mice and severe hypoxia, cold, fatigue stress in mice have a good stress response. Conclusion: In this study, immunization and stress experiments in mice showed that the prescription of promoting blood circulation and nourishing blood, which was formulated under the guidance of the principle of strengthening the body and dispelling the evil and expelling evil, can improve the immune function and regulate the stress response in mice, It is instructive to explore the objectification of the rule of the motherland and eliminate the evil in the motherland.