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目的 观察利多卡因对 N-甲基-D-天冬氨酸(NMDA)抑制大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)增殖的影响。方法 将体外培养的 PC12细胞分为6组，分别采用正常不含药液的培养基(C组)；含400μmol·L(-1)NMDA 的培养基(N组)；NMDA 分别混合10μmol·L~(-1)(L_1组)、10~2μmol·L~(-1)(L_2组)、10~3μmol·L~(-1)(L_3组)以及10~4μmol·L~(-1)(L_4组)利多卡因的培养基培养5d，应用流式细胞仪测定细胞 DNA 相对含量，解析细胞周期，计算 S 期细胞荧光强度占受测细胞总荧光强度的百分数为 S期分数(SPF)和 S 期与 G_2期细胞荧光强度之和与 M 期细胞荧光强度的比值[(S+G_2)/M]。结果 与C 组比较，N、L_1组 SPF 和(S+G_2)/M 均降低(P<0.05)，L_4组 SPF 降低(P<0.05)，而 L_2及 L_3组 SPF和(S+G_2)/M 差异无统计学意义(P>0.05)。与 N 组比较，L_2、L_3及 L_4组 SPF 和(S+G_2)/M 升高(P<0.05)，L_1组 SPF 升高(P<0.05)，而(S+G_2)/M 差异无统计学意义(P>0.05)。结论 NMDA 可以通过抑制 PC12细胞 DNA 合成而影响细胞的增殖活性，利多卡因能拮抗 NMDA 对 PC12细胞增殖的抑制作用。
Objective To observe the effect of lidocaine on the proliferation of adrenal pheochromocytoma cells (PC12 cells) induced by N-methyl-D-aspartate (NMDA) in rats. Methods PC12 cells cultured in vitro were divided into 6 groups: normal saline-free medium (group C), medium containing 400 μmol·L -1 NMDA (group N), and NMDA supplemented with 10 μmol·L L_1 group, L_2 group, L_2 group, L_3 group, L_3 group, L_2 group), L_2 group (10μmol·L -1) (L_4 group) were cultured for 5 days. The relative content of DNA was determined by flow cytometry, and the cell cycle was analyzed. The percentage of S phase fluorescence intensity in total cells was calculated as S phase fraction (SPF) And the ratio of the sum of the fluorescence intensity of S phase and G 2 phase cells to the fluorescence intensity of M phase cells [(S + G 2) / M]. Results Compared with group C, the SPF and (S + G 2) / M of N and L 1 groups decreased (P <0.05), and the SPF of L 4 group decreased (P <0.05) M difference was not statistically significant (P> 0.05). Compared with N group, the SPF and (S + G_2) / M of L_2, L_3 and L_4 groups increased (P <0.05), while that of L_1 group increased (P <0.05) Significance (P> 0.05). Conclusion NMDA can affect the proliferation activity of PC12 cells by inhibiting DNA synthesis. Lidocaine can antagonize the inhibitory effect of NMDA on the proliferation of PC12 cells.