化学致癌物通常需经酶的活化才显示出生物效应。但很多类型的细胞并不能对前致癌物起活化作用,对于这一类细胞的诱变作用是通过加入具有酶活性的肝细胞组分来进行的,但肝亚细胞组分对某些致癌物[例如黄曲霉毒素B_1(AF)、3.4-苯并芘(BP)、7.12-二甲基苯蒽]的代谢产物及其与 DNA 结合产物在类型上与完整细胞并不相同,它不能模拟活体的真实情况。 Chemical carcinogens usually require the activation of enzymes to show biological effects. However, many types of cells do not activate pre-carcinogens. Mutagenesis of this type of cells is carried out by adding a hepatocyte component that has enzyme activity, but the sub-cellular components of the liver are specific to certain carcinogens. [For example, the metabolites of aflatoxin B_1 (AF), 3.4-benzopyrene (BP), 7.12-dimethylbenzoquinone] and their DNA-binding products are not the same in type as intact cells, and they cannot mimic living organisms. The real situation.