论文部分内容阅读
目的为临床制定安全有效的给药方案提供参考。方法采用液相色谱-串联质谱(LC-MS-MS)法。血浆样品经液-液萃取处理后,以乙腈-水(体积比25∶75)为流动相,采用Zorbax Ec lipseXDB C8柱(150 mm×4.6 mm,5μm)分离;通过电喷雾三重四级杆串联质谱,以负离子、选择反应监测方式进行检测,用于定量的离子反应分别为m/z452→m/z311(氟氯西林)和m/z468→m/z327(内标双氯西林)。结果氟氯西林线性范围为20.0~15000μg.L-1,定量下限为20.0μg.L-1,提取回收率均大于70%,日内、日间精密度均小于7%。结论该法适用于注射用氟氯西林钠的单次肌内注射给药的药物动力学研究。
Objective To provide a safe and effective dosing regimen for clinical reference. Methods Liquid chromatography-tandem mass spectrometry (LC-MS-MS) was used. The plasma samples were separated by liquid-liquid extraction using acetonitrile-water (25:75 by volume) as a mobile phase using a Zorbax EciplexXDB C8 column (150 mm × 4.6 mm, 5 μm); electrospray triple quadrupole Mass spectrometry was performed with negative ions and selective reaction monitoring. The ion reactions for quantification were m / z 452 → m / z 31 1 (flucloxacillin) and m / z 468 → m / z 327 (internal standard dicloxacillin), respectively. Results The linear range of flucloxacillin was 20.0 ~ 15000μg.L-1, the lower limit of quantification was 20.0μg.L-1, the recoveries were both higher than 70%. The intra-day and inter-day precision were less than 7%. Conclusion This method is suitable for the pharmacokinetic study of a single intramuscular injection of flucloxacillin sodium for injection.