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目的 了解姜黄素与阿霉素联合应用对人白血病K5 6 2细胞及人肝癌Bel 740 2细胞的杀伤作用。方法 采用MTT法测定药物的体外杀伤作用 ,应用金氏公式进行联合用药分析。结果 姜黄素 2 0 4μmol·L-1～ 16 2 9μmol·L-1,阿霉素 0 70 μmol·L-1～ 5 5 2 μmol·L-1同时给药对K5 6 2细胞及Bel740 2细胞可产生单纯相加至增强的协同杀伤效果。序贯给药法中先给姜黄素后给阿霉素结果为协同作用 ,先给阿霉素后给姜黄素为拮抗作用。结论 姜黄素与阿霉素同时联合用药可产生协同作用 ,序贯联合用药产生单向协同作用。
Objective To investigate the killing effect of curcumin combined with adriamycin on human leukemia K562 cells and human hepatoma Bel 740 2 cells. Methods The in vitro killing effect of the drug was measured by MTT assay. The Kim’s formula was used for the analysis of combined drug use. Results Curcumin was 2-4 μmol·L-1 to 16 29 μmol·L-1, and doxorubicin 0 70 μmol·L-1 to 5 52 μmol·L-1 was administered simultaneously to K562 cells and Bel740 2 cells. It can produce a purely additive to enhanced synergistic killing effect. The results of sequential administration of curcumin after administration of doxorubicin were synergistic, giving curcumin after doxorubicin was antagonistic. Conclusion The combined use of curcumin and doxorubicin at the same time can produce a synergistic effect, and the sequential combination of drugs can produce a one-way synergistic effect.