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采用聚合酶链反应（ＰＣＲ）方法获得小鼠抗人小细胞肺癌单克隆抗体的变区基因（ｃＤＮＡ），构建成含人──鼠抗人小细胞肺癌嵌合重链基因质粒（ｐＳＶ＿２△Ｈｇｐｔ２Ｆ＿７Ｖ＿ＨＨｕγ＿３）ＤＮＡ通过电脉冲介导基因转移的方法，转染小鼠骨髓瘤细胞Ｊ５５８Ｌ，并经选择培养基筛选出含有构建质粒基因的克隆，进一步按ＥＬＩＳＡ方法测定细胞表面和培养上清液中嵌合重链抗体的表达效率，最终选出表达抗体效率较高的单克隆细胞株为５Ｃ＿２，１２Ｂ＿９，２９Ｃ＿６。Ｎｏｒｔｈｅｒｎｂｌｏｔ方法检测证明，阳性克隆细胞总ＲＮＡ经电泳后在１８Ｓ附近处与鼠抗人小细胞肺癌单抗的重链变区ｃＤＮＡ探针有明显的阳性杂交带出现。细胞抽提蛋白经ＳＤＳ──ＰＡＧＥ电泳分离显示在正常人ＩｇＧ重链分子相当的位置有一清晰条带出现，分子量约为５８ＫＤ，免疫印迹方法同样检测出此条带特异他与兔抗人ＩｇＧ（重链特异）抗体反应，证实人──鼠抗人小细胞肺癌嵌合重链基因在小鼠骨髓癌细胞中表达成功。
The mouse murine anti-small cell lung cancer monoclonal antibody variant (cDNA) was obtained by polymerase chain reaction (PCR) method to construct a human-mouse anti-small cell lung cancer chimeric heavy chain gene plasmid (pSV_2ΔHgpt2F_7V_HHuγ_3 ) DNA was transfected into mouse myeloma cell line J558L by electroporation-mediated gene transfer, and a clone containing the constructed plasmid gene was selected in the selection medium, and the cell surface and the culture supernatant were further determined by ELISA. The efficiency of heavy chain antibody expression, the final selection of monoclonal antibody cell lines with higher antibody expression efficiency 5C_2, 12B_9, 29C_6. Northern blot analysis showed that the positive cloned cells showed significant positive hybridization bands with the heavy chain variable region cDNA probe of mouse anti-small cell lung cancer monoclonal antibody after 18S. The cell-extracted protein was separated by SDS-PAGE electrophoresis and showed a clear band at the position corresponding to the normal human IgG heavy chain molecule. The molecular weight was about 58KD. Western blotting method also detected this band specific he rabbit anti-human IgG ( The heavy chain specific) antibody response confirmed that the human mouse anti-small cell lung cancer chimeric heavy chain gene was successfully expressed in mouse bone marrow cancer cells.