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目的:探讨子宫内膜癌患者P16基因甲基化和血清CA125检测的临床意义。方法:采用DNA甲基化特异性PCR分别对46例子宫内膜癌及12例正常内膜对照组织中抑癌基因p16甲基化进行检测;对所有病例术前均测定血清CA125水平。结果:子宫内膜癌组p16甲基化率45.6%,而12例对照组织均未检测到甲基化;子宫内膜癌组血清CA125水平明显升高。结论:子宫内膜癌患者p16基因甲基化和血清CA125水平与发病关系密切,两者结合对早期诊断及预后判断具有一定的临床指导意义。
Objective: To investigate the clinical significance of methylation of P16 gene and serum CA125 in patients with endometrial carcinoma. Methods: Methylation of tumor suppressor gene p16 in 46 cases of endometrial carcinoma and 12 cases of normal endometrium was detected by DNA methylation-specific PCR. Serum CA125 level was measured in all cases before operation. Results: The methylation rate of p16 in endometrial carcinoma group was 45.6%, while no methylation was detected in 12 cases. Serum CA125 level in endometrial carcinoma group was significantly higher than that in endometrial carcinoma group. Conclusion: The methylation status of p16 gene and the level of serum CA125 in endometrial cancer patients are closely related to the pathogenesis. The combination of the two can be of clinical significance in early diagnosis and prognosis.