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目的:复制小鼠慢性非细菌性前列腺炎(CAP)模型,研究前列腺炎发展过程中α1肾上素能受体(α1-AR)亚型的表达及其与前列腺中炎症介质TNF-α的关系。方法:使用大鼠前列腺蛋白提纯液辅以双重免疫佐剂,造成实验性慢性非细菌性前列腺炎(CAP)小鼠模型,在造模后45天解剖小鼠,获得前列腺组织样本,抽提RNA转录为cDNA,运用实时荧光逆转录聚合酶链反应检测组织标本中α1a-AR、α1b-AR、α1d-AR、TNF-α的mRNA的表达,观察CAP小鼠α1-AR三种亚型的表达变化,并观察其与前列腺中炎症介质TNF-α的关系。背侧组织做病理切片观察,进一步判别前列腺的炎症程度。结果:造模小鼠均出现排尿障碍等症状;病理切片观察到炎症细胞浸润;造模小鼠中TNF-α明显升高,但α1a-AR、α1b-AR、α1d-AR无明显变化。结论:慢性前列腺炎小鼠模型复制成功,CAP的炎症程度与TNF-α的表达成正相关关系,与α1a-AR、α1b-AR、α1d-AR无明显关系。
OBJECTIVE: To replicate chronic nonbacterial prostatitis (CAP) model in mice and study the expression of α1-adrenoceptor (α1-AR) subtype in prostatitis and its relationship with inflammatory mediator TNF-α in prostate . METHODS: Experimental chronic nonbacterial prostatitis (CAP) mouse model was established by using rat prostatic protein extract supplemented with double immunological adjuvant, and mice were dissected 45 days after model establishment to obtain prostate tissue samples. RNA was extracted Transcribed into cDNA. The expression of α1a-AR, α1b-AR, α1d-AR and TNF-α mRNA in tissue samples was detected by real-time fluorescence reverse transcription polymerase chain reaction Changes and to observe its relationship with the inflammatory mediator TNF-α in the prostate. Dorsal tissue sections were observed, to further determine the degree of inflammation of the prostate. Results: The model mice showed symptoms such as dysuria. Inflammatory cell infiltration was observed in the pathological sections. TNF-α in model mice was significantly increased, but there was no significant change in α1a-AR, α1b-AR and α1d-AR. CONCLUSION: The model of chronic prostatitis is successfully replicated in mice. The degree of inflammation of CAP is positively correlated with the expression of TNF-α, and has no significant relationship with α1a-AR, α1b-AR and α1d-AR.