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目的观察调肝解毒方对慢性点燃癫痫大鼠情感行为的影响及其作用机制。方法腹腔注射印防己毒素制作慢性癫痫模型,将造模成功的60只SD大鼠随机分为模型组、调肝低量组、调肝高量组、拉莫三嗪组各15只,另设正常组(15只)同期观察治疗60天。治疗期间正常组、模型组灌胃10ml/kg生理盐水;调肝低量组、调肝高量组分别灌胃0.4g/kg、0.8g/kg调肝解毒方混悬液;拉莫三嗪组灌胃0.002g/kg拉莫三嗪混悬液,每天1次。用旷场实验、抬高迷宫实验观察大鼠情感行为改变,并检测大鼠海马等脑组织神经肽Y(NPY)的表达。结果与模型组比较,拉莫三嗪组、调肝高量组情感行为学各指标差异均有统计学意义(P<0.05或P<0.01);调肝低量组拒俘反应性评分及开放臂逃避时间差异亦有统计学意义(P<0.05)。与模型组比较,其他各组大鼠脑海马区、尾壳核NPY蛋白表达均明显提高(P<0.05或P<0.01);拉莫三嗪组、调肝高量组脑海马各区NPY阳性细胞数均有增加(P<0.05或P<0.01);调肝低量组CA3、DG区亦有增加(P<0.01)。结论调肝解毒方可改善慢性点燃癫痫大鼠情感行为异常,并可能与其调节脑组织NPY表达有关。
Objective To observe the effect of Tiaogan Jiedu Fang on emotional behavior in chronic ignite epilepsy rats and its mechanism. Methods The model of chronic epilepsy was established by intraperitoneal injection of picrotoxin. Sixty SD rats were randomly divided into model group, low-dose liver-regulating group, high-dose liver-regulating group and lamotrigine group. Normal group (15) observed the same period for 60 days. The normal group during the treatment, the model group fed with 10ml / kg saline; low liver regulating group, high liver regulating group were fed 0.4g / kg, 0.8g / kg Tiaogan Jiedu suspension; Group orally stomach 0.002g / kg lamotrigine suspension, 1 times a day. The open field test and elevation maze test were used to observe the changes of emotional behavior, and the neuropeptide Y (NPY) expression in hippocampus and other brain tissues of rats was detected. Results Compared with the model group, there were significant differences in emotional behavior between the lamotrigine group and the control group (P <0.05 or P <0.01) Arm evasion time was also statistically significant (P <0.05). Compared with the model group, NPY protein expression in hippocampus and caudate putamen of the other groups were significantly increased (P <0.05 or P <0.01). Compared with model group, NPY positive cells in hippocampus (P <0.05 or P <0.01). The levels of CA3 and DG in the low-dose group also increased (P <0.01). Conclusion Tiaogan Jiedu decoction can improve the emotional behavior of chronic ignition epilepsy rats, and may be related to its regulation of brain tissue NPY expression.