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目的:制备麻醉犬急性心肌梗死(AMI)模型,观察参附注射液对急性心肌梗死犬心肌酶学和氧摄取率的影响,初步探讨其对AMI的保护作用及可能的作用机制。方法:采用结扎冠脉法建立犬AMI模型。将健康杂种犬随机分为阴性对照组,丹参注射液组,参附注射液大、中、小剂量组,于冠脉结扎后15 min各组犬经股静脉滴注相应药物,结扎后30,60,90,120及180 min取血测氧摄取率,测定各组犬冠脉结扎前和结扎后180 min时血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)以及丙二醛(MDA)的含量。结果:冠脉结扎后,阴性对照组犬血清CK、LDH和MDA含量显著升高,SOD含量明显减少,氧摄取率显著降低。与阴性对照组比较,丹参组和参附注射液各剂量组能显著对抗血清中CK、LDH和MDA活性的增高以及SOD、氧摄取率的降低。结论:参附注射液对犬AMI有一定的保护作用。
Objective: To study the effect of Shenfu injection on myocardial enzymology and oxygen uptake in dogs with acute myocardial infarction (AMI), and to explore its protective effect on AMI and its possible mechanism. Methods: AMI model of canine was established by ligation of coronary artery. The healthy mongrel dogs were randomly divided into negative control group, Danshen injection group, Shenfu injection large, medium and small dose groups. After 15 minutes of coronary ligation, At 60, 90, 120 and 180 min, blood oxygen uptake rate was measured and the levels of creatine kinase (CK), lactate dehydrogenase (LDH), superoxide dismutase (SOD) SOD) and malondialdehyde (MDA) content. Results: After coronary ligation, serum CK, LDH and MDA in the negative control group were significantly increased, the content of SOD was significantly decreased and the oxygen uptake rate was significantly decreased. Compared with the negative control group, each dose group of Salviae Miltiorrhizae and Shenfu Injection significantly antagonized the increase of serum CK, LDH and MDA activity as well as the decrease of SOD and oxygen uptake rate. Conclusion: Shenfu injection has certain protective effect on AMI in dogs.