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利用cDNA-AFLP技术分析了茶树在干旱胁迫诱导下的基因表达差异,通过256对引物组合共获得27个差异表达片段(TDF),通过BLAST比对分析,按其功能分为转录因子、基础代谢相关蛋白、抗逆蛋白、信号转导蛋白,此外还有一些假设蛋白、未知蛋白和没有比对的基因片段。同时利用RT-PCR对片段GH2和GH15进行验证,表明GH2和GH15片段均受到干旱胁迫诱导表达。这些研究结果显示茶树在干旱胁迫下的逆境反应非常复杂,涉及多种代谢过程中的众多基因。
A total of 27 differentially expressed fragments (TDFs) were obtained from 256 pairs of primers by cDNA-AFLP analysis. The results of BLAST analysis showed that TDFs were classified into transcription factors, basal metabolism Related proteins, anti-inversion proteins, signal transduction proteins, in addition to some putative proteins, unknown proteins and unaligned gene fragments. At the same time, the fragments GH2 and GH15 were verified by RT-PCR, indicating that the GH2 and GH15 fragments were all induced by drought stress. These findings show that the stress response of tea plants under drought stress is complex and involves many genes involved in a variety of metabolic processes.