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用重组人粒细胞－巨噬细胞集落刺激因子－白细胞介素３（ｒｂＧＭ－ＣＳＦ－ＩＬ３）融合蛋白（Ｇ３）作为免疫原．通过Ｂ淋巴细胞杂交瘤技术建立了两株稳定分泌抗ｒｈＧＭ－ＣＳＦ－ＩＬ３（Ｇ３）单克隆抗体的杂交瘤细胞株．分别命名为ＭＧ３１和ＭＧ３２。经鉴定，两种单抗均为小鼠ＩｇＧ１亚类。其染色体众数分别为９１和８６．并能特异地识别分子量约３０ＫＤ的Ｇ３。ＭＧ３１和ＭＧ３２经３个月传代培养及冻存复苏．在培养沿及Ｂａｌｂ／ｃ小鼠腹水中均能产生而滴度抗体。抗Ｇ３单克隆抗体的获得为深入研究Ｇ３的亲和层析纯化、Ｇ３的免疫学测定及其生物学特性提供了必要的条件。
Recombinant human granulocyte-macrophage colony stimulating factor-interleukin-3 (rbGM-CSF-IL3) fusion protein (G3) was used as an immunogen. Two hybridoma cell lines stably secreting anti-rhGM-CSF-IL3 (G3) monoclonal antibody were established by B lymphocyte hybridoma technique. Named MG31 and MG32 respectively. Identified, the two monoclonal antibodies are mouse IgG1 subclass. The chromosomes were 91 and 86 respectively. And can specifically identify G3 with a molecular weight of about 30KD. MG31 and MG32 after 3 months subculture and cryopreservation. Antibodies were titrated in both ascites and Balb / c mice. The obtaining of anti-G3 monoclonal antibody provided necessary conditions for in-depth study on purification of G3 by affinity chromatography, immunological determination of G3 and its biological characteristics.