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Five new polyhydroxylated furostanol saponins were isolated from the roots and rhizomes of Tupistra chinensis,and their structures were determined as tupistrosides J-N (1-5),together with four known furostanol saponins (6-9),on the basis of physico-chemical properties and spectral analysis.Among them,compounds 3 and 5 showed cytotoxicity against human cancer cell lines SW620 with IC50 values of 72.5 ± 2.4 and 77.3 ± 2.5 μmol·L-1,respectively.Compound 4 showed cytotoxicity against human cancer cell line HepG2 with IC50 value of 88.6 ± 2.1 μmol.L-1.