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目的分析福建省2015年一起流行性腮腺炎暴发的病原学特征及基因变异情况。方法通过Vero/Slam细胞进行病毒分离;采用逆转录-聚合酶链反应(RT-PCR)扩增小疏水蛋白基因片段进行病毒鉴定;应用序列分析软件对病毒基因特征进行比较分析。结果成功分离到9株腮腺炎病毒,均属于G基因型。9株分离株核苷酸序列同源性为99.0%~100%,氨基酸序列同源性为98.0%~100%;与国内其他省流行的F基因型代表株核苷酸与氨基酸差异均>13.0%;与疫苗株的核苷酸及氨基酸平均差异分别为15.3%和34.5%;同2003年流行于英国的G2亚型病毒代表株核苷酸序列同源性最高(96.8~97.2%)。腮腺炎病毒分离株SH基因的核苷酸存在8个特异性突变位点,其中CNT68、ANT199、TNT271是新发现的突变位点;部分氨基酸保守位点也发生了变化。结论此起暴发是由G基因型腮腺炎病毒引起的。
Objective To analyze the etiological characteristics and genetic variation of a mumps outbreak in Fujian Province in 2015. Methods Vero / Slam cells were used for virus isolation. Small hydrophobic protein gene fragments were amplified by reverse transcription-polymerase chain reaction (RT-PCR) for virus identification. Sequence analysis software was used to analyze the characteristics of the virus genes. Results Nine strains of mumps were successfully isolated and belonged to G genotype. The homologies of nine isolates were 99.0% ~ 100% and the homologies of amino acid sequences were 98.0% ~ 100%. The nucleotide and amino acid identities of the representative strains of F genotypes prevailing in other provinces in China were all> 13.0 %; The average nucleotide and amino acid difference with the vaccine strain was 15.3% and 34.5% respectively. The nucleotide sequence and amino acid identity with the vaccine strain in 2003 was the highest (96.8-97.2%). Mumps virus isolated SH gene nucleotide there are eight specific mutations in which CNT68, ANT199, TNT271 is a newly discovered mutation sites; part of the amino acid conserved sites also changed. Conclusion This outbreak was caused by the G genotype mumps virus.