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目的探讨血浆血小板源生长因子(PDGF-BB)水平在肺结核病患者与健康人之间是否具有显著差异,以及其水平与患者载菌量是否相关。方法收集134例初诊活动性肺结核病患者的EDTA抗凝血浆,并根据痰涂片及痰培养结果进行分组:涂片培养阳性组(S+/C+)55例,涂片阴性培养阳性组(S-/C+)43例,涂片培养阴性组(S-/C-)36例;健康对照组为健康体检40例。应用MILLIPLEXMAP Kit细胞因子试剂盒检测血浆中PDGF-BB的水平。结果结核病组血浆PDGF-BB水平显著高于健康对照组,差异有统计学意义(P<0.05);S+/C+组血浆PDGF-BB水平显著高于S-/C-组的测定值,差异有统计学意义(P<0.05);S+/C+组血浆PDGF-BB水平显著高于健康对照组,差异有统计学意义(P<0.01)。ROC曲线的AUC值为0.540,当临界值为271.4时,敏感度为28.6%,特异度为95.0%。结论 PDGF-BB水平与结核分枝杆菌感染及机体载菌量存在一定相关性,可能作为肺结核病患者临床辅助诊断的标志物之一,但是需要与其他细胞因子联合检测提高检测性能。
Objective To investigate whether there is a significant difference in the level of platelet-derived growth factor (PDGF-BB) between patients with pulmonary tuberculosis and healthy people and whether their levels are related to the amount of bacteria in patients. Methods Totally 134 anticoagulated plasma samples from patients with newly diagnosed active pulmonary tuberculosis were collected and divided into sputum smear and sputum according to the results of smear culture positive group (S + / C +) 55 cases and smear negative culture positive group (S- / C +) 43 cases, smear negative group (S- / C-) 36 cases; healthy control group of 40 healthy physical examination. The level of PDGF-BB in plasma was measured using the MILLIPLEX ™ MAP Kit cytokine kit. Results The level of plasma PDGF-BB in tuberculosis group was significantly higher than that in healthy control group (P <0.05). The level of PDGF-BB in S + / C + group was significantly higher than that in S- / C-group (P <0.05). The plasma level of PDGF-BB in S + / C + group was significantly higher than that in healthy control group (P <0.01). The AUC of ROC curve was 0.540. When the critical value was 271.4, the sensitivity was 28.6% and the specificity was 95.0%. Conclusions The level of PDGF-BB is correlated with Mycobacterium tuberculosis infection and the amount of bacteria in the bacterium. It may be one of the markers of clinical diagnosis in patients with pulmonary tuberculosis. However, it needs to be combined with other cytokines to improve the detection performance.