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探讨播散性血管内凝血（ＤＩＣ）时循环血中血浆白细胞介素８（ＩＬ８）的动态变化规律及其可能的发生机制。采用间隔２４小时两次静脉注射内毒素（ＬＰＳ）方法制作家兔内毒素性ＤＩＣ模型。在模型复制前后不同时间采用夹心酶联免疫吸附法（ＥＬＩＳＡ）测定ＩＬ８水平；同时观察了外周血白细胞（ＷＢＣ）和中性粒细胞（ＰＭＮ）及脏器组织的病理变化。实验发现：在第１次注射ＬＰＳ后２小时血浆ＩＬ８出现峰值（８３９．８１±５６．６１ｎｇ／Ｌ），其次为第２次注射ＬＰＳ后２小时ＩＬ８为６５４．３７±６８．３８ｎｇ／Ｌ，且呈平坦的单峰曲线，持续时间较长（６小时），均较实验前（４２．９４±１３．８５ｎｇ／Ｌ）明显增高（Ｐ＜０．０１）。ＩＬ８与ＷＢＣ和ＰＭＮ计数变化密切相关（ｒ＝０．８０９，Ｐ＜０．０１；ｒ＝０．７９５，Ｐ＜０．０１）。作者认为：ＩＬ８参与内毒素性ＤＩＣ的发生，确定机体受内毒素攻击后释放ＩＬ８的时机，适时应用拮抗剂，将具有重要的临床意义
To investigate the dynamic changes of circulating interleukin 8 (IL-8) and its possible mechanism in disseminated intravascular coagulation (DIC). Rabbit endotoxin DIC model was made by intravenous injection of endotoxin (LPS) twice a day. IL8 levels were measured by sandwich enzyme-linked immunosorbent assay (ELISA) at different time points before and after model replication. Pathological changes of peripheral blood leukocytes (WBC), neutrophils (PMN) and visceral tissues were also observed. The results showed that the plasma IL8 peaked at 2 hours after the first injection of LPS (839.81 ± 56.61 ng / L), followed by 654.37 ± 68.38 ng / L at 2 hours after the second injection of LPS, (P <0.01), which showed a flat single peak curve with a longer duration (6 hours) than that before the experiment (42.94 ± 13.85 ng / L). IL8 was closely related to WBC and PMN count changes (r = 0.809, P <0.01; r = 0.795, P <0.01). The authors believe that: IL8 involved in the occurrence of endotoxic DIC, to determine the body after endotoxin challenge release IL8 timing, timely application of antagonists, will have important clinical implications