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目的:检测人肺癌组织中Atrogin-1基因mRNA表达水平,了解对肺癌患者术后生存情况及其影响因素。方法:运用实时定量PCR技术测定60例肺癌患者的癌组织及配对的癌旁组织中Atrogin-1基因mRNA表达水平,采用Kaplan-Meier单因素与Cox多元回归进行生存分析。结果:肺癌组织中Atrogin-1 mRNA表达水平(-8.15±3.94)显著高于其配对的癌旁组织(-10.39±4.35)(t=3.873,P=0.000),其中71.67%(43/60)患者Atrogin-1 mR-NA高表达,28.33%(17/60)患者低表达。Kaplan-Meier法生存分析结果显示:Atrogin-1的表达水平、淋巴结转移、家族史、病理分级与患者的生存率显著相关。Cox回归分析结果显示:Atrogin-1 mRNA表达水平、淋巴结转移、病理分级和吸烟是影响肺癌患者生存率的独立危险因素。结论:肺癌组织中Atrogin-1的高表达可能影响肺癌患者术后的生存,其可作为肺癌患者预后的一个评价指标,并可能成为基因治疗的一个作用靶点。
OBJECTIVE: To detect the mRNA expression of Atrogin-1 in human lung cancer and to investigate the postoperative survival and its influencing factors in patients with lung cancer. Methods: Real-time PCR was used to detect the mRNA expression of Atrogin-1 in 60 cases of lung cancer tissues and matched paracancerous tissues. Survival analysis was performed by Kaplan-Meier single factor and Cox multiple regression. Results: The expression of Atrogin-1 mRNA in lung cancer tissues (-8.15 ± 3.94) was significantly higher than that in paired paracancer tissues (-10.39 ± 4.35, t = 3.873, P = 0.000), of which 71.67% Patients with Atrogin-1 mR-NA high expression, 28.33% (17/60) patients with low expression. Survival analysis of Kaplan-Meier method showed that the expression of Atrogin-1, lymph node metastasis, family history and pathological grade were significantly correlated with the survival rate of patients. Cox regression analysis showed that Atrogin-1 mRNA expression, lymph node metastasis, pathological grading and smoking were independent risk factors affecting the survival rate of patients with lung cancer. Conclusion: The high expression of Atrogin-1 in lung cancer may affect the postoperative survival of patients with lung cancer, which may be used as a prognostic indicator of lung cancer patients and may be a target of gene therapy.