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目的:探讨早期药膳饮食对严重烫伤大鼠肠道免疫屏障的保护作用。方法:设计一种药膳饮食,按照药膳饮食的制作方法熬制成汤剂。Wistar大鼠100只,随机分成药膳喂养组(药膳组30只),肉汤喂养组(肉汤组30只),常规喂养组(模型组30只),正常对照组(正常对照组10只)。前三组均给予背部30%TBSAⅢ度烫伤,伤后早期分别给予药膳+饲料、肉汤+饲料和单纯饲料喂养,于伤后第3、7、14天各取10只,无菌条件下取材检测:(1)肠黏膜病理形态;(2)血浆内毒素含量;(3)空回肠黏液中SIgA(分泌型免疫球蛋白A)含量。正常对照组为37℃假伤,并于实验开始后立即处死取材送检。结果:(1)药膳组、肉汤组、模型组大鼠伤后肠黏膜发生了不同的病理改变。药膳组伤后第14天已基本恢复正常,肉汤组、模型组在伤后第14天仍未完全恢复正常。(2)与正常对照组比较,肉汤组、药膳组、模型组伤后各时相点血浆内毒素显著上升(P<0.05或P<0.01);药膳组与肉汤组、模型组比较,伤后各时相点均低于肉汤组、模型组(P<0.05或P<0.01)。(3)与正常对照组比较,药膳组伤后第3、7天SI-gA显著降低(P<0.05),但伤后第14天,药膳组与正常对照组比较无统计学意义(P>0.05);肉汤组、模型组各时相点SIgA的浓度明显低于正常对照组(P<0.05或P<0.01);药膳组与肉汤组、模型组比较,药膳组伤后各时相点均显着高于肉汤组、模型组(P<0.05或P<0.01)。结论:早期药膳喂养可促进烫伤大鼠肠道黏膜的恢复,降低了血浆内毒素含量,促进SIgA分泌。药膳喂养组优于肉汤喂养组和常规喂养组。
Objective: To explore the protective effect of early diet on gut immune barrier in severely scalded rats. Methods: Design a medicated diet, according to the method of making medicated diet boil decoction. 100 Wistar rats were randomly divided into three groups: Diet group (30 Diet group), Broth group (30), Normal group (30), Normal group (Normal group) . The first three groups were given 30% TBSA Ⅲ degree scald on the back. The rats were given medicated diet + feed, broth + feed and simple feed respectively in the early stage, 10 in the 3rd, 7th and 14th day after the injury, Detection: (1) pathological morphology of intestinal mucosa; (2) plasma endotoxin content; (3) Ileum intestinal mucus SIgA (secreted immunoglobulin A) content. The normal control group was a 37 degree pseudosia and was sacrificed immediately after the start of the experiment. Results: (1) Different pathological changes occurred in the intestinal mucosa of the medicated group, the broth group and the model group. Diet group 14 days after injury has returned to normal, the broth group, the model group 14 days after injury has not fully returned to normal. (2) Compared with the normal control group, the levels of plasma endotoxin in the broth group, the diet group and the model group at each time point after injury significantly increased (P <0.05 or P <0.01); Compared with the broth group and the model group, The time points after injury were lower than that of the broth group and the model group (P <0.05 or P <0.01). (3) Compared with the normal control group, SI-gA decreased significantly on the 3rd and 7th day in the Diet group (P <0.05), but on the 14th day after injury, there was no significant difference between the Diet group and the normal control group (P> 0.05). The concentration of SIgA at each time point in the broth group and the model group was significantly lower than that in the normal control group (P <0.05 or P <0.01). Compared with the broth group and the model group, Points were significantly higher than the broth group, model group (P <0.05 or P <0.01). Conclusion: The early diet can promote the recovery of intestinal mucosa in scalded rats, reduce the content of plasma endotoxin and promote the secretion of SIgA. Medicated feeding group was better than the broth feeding group and conventional feeding group.