The perennial Medicago sativa cv.Gabès is widely grown on saline soils in Tunisian oases.Morphological and physiological analyses of two populations (Mareth and Gannouch) were conducted in order to study the efiect of salinity and gamma radiation (350 Gy) inter-action on two populations of this species.It has been shown that the two irradiated populations of Medicago sativa are fairly tolerant to salt at growth phase compared to the non-irradiated.Exposure to gamma irradiation (350 Gy), alone or in combination with salt stress, increased significantly (p < 0.001) shoot number, stem height and chlorophyll b pigment especially for the Gannouch population, while no change occurred for the Mareth population.The presence of salt to 9 g/l afiected significantly the root biomass and induced a reduc-tion of shoot development of both control and irradiated alfalfa populations.For all treatments, plants of two populations remained able to produce and to allocate dry matter to the difierent organs.The survey of Na+/K+ ratio showed that the growth of the aerial organs of two non-irradiated populations was at least determined by a selectivity in favour of the K+ ions (r2 = 0.97 and r2 = 0.59 for Mareth and Gannouch non-irradiated populations, respectively).However, the rather weak correlation detected for the irradiated populations, par-ticularly for the irradiated Gannouch, seems to be the consequence of the efiect of irradiation that improved potassium availability, essential element for growth and development.Results also showed that the two irradiated populations, especially the Mareth, accumu-lated Na+ ions in its photosynthetic organs.This accumulation was associated with an improvement of foliar water content at a level of salinity around 5 g/l.Such a mechanism refiects probably an inclusive behaviour of the plants and a good aptitude to use the dominant ions (Na+) for the osmotic adjustment.However, the non-irradiated populations are unable to adjust their internal osmotic potential.Consequently, irradiated plants have probably adapted to the osmotic stress by either closing their stomata or increasing the osmotic pressure of the leaf cells. The perennial Medicago sativa cv. Gabès is widely grown on saline soils in Tunisian oases. Morphological and physiological analyzes of two populations (Mareth and Gannouch) were conducted in order to study the efiect of salinity and gamma radiation (350 Gy) inter-action on Two populations of this species. It has been shown that the two irradiated populations of Ipatotica are fairly tolerant to salt at growth phase compared to the non-irradiated. Expose to gamma irradiation (350 Gy), alone or in combination with salt stress, increased significantly (p <0.001) shoot number, stem height and chlorophyll b pigment especially for the Gannouch population, while no change occurred for the Mareth population. The presence of salt to 9 g / l afiected significantly the root biomass and induced a reduc- tion of shoot development of both control and irradiated alfalfa populations. For all treatments, plants of two seeds who able to produce and to allocate dry matter to the difierent orga ns.The survey of Na + / K + ratio showed that the growth of the aerial organs of two non-irradiated populations was at least determined by a selectivity in favor of the K + ions (r2 = 0.97 and r2 = 0.59 for Mareth and Gannouch non- irradiated populations, respectively). However, the rather weak correlation detected for the irradiated populations, par-ticularly for the irradiated Gannouch, seems to be the consequence of the efiect of irradiation that improved potassium availability, essential element for growth and development. Results also showed that the two irradiated populations, especially the Mareth, accumu-lated Na + ions in its photosynthetic organs. This accumulation was associated with an improvement of foliar water content at a level of salinity around 5 g / l. Such a mechanism refiects may an inclusive behavior of the plants and a good aptitude to use the dominant ions (Na +) for the osmotic adjustment. However, the non-irradiated populations are unable to adjust their internal osmo tirradiated plants have probably adapted to the osmotic stress by either closing their stomata or increasing the osmotic pressure of the leaf cells.