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鼠疫是一种历史上曾给人类带来巨大灾难的传染性疾病。进入90年代,人间鼠疫的发病率呈上升趋势。目前对这种疾病的疫区判定仍然是在常规检查(显微镜检查、培养、噬菌体裂解试验及动物实验)基础上,发展了被动血凝试验及其它酶联免疫吸附实验(ELISA),但这些方法在可靠性和简便程度方面仍存在一些问题。因此,建立高度敏感、快速检测鼠疫菌的方法仍是急迫和必要的。 为了更可靠地利用核酸技术,特别是在难于分离病原体的情况下,直接对疫源地中的鼠疫疫情进
The plague is a contagious disease that has historically caused enormous human catastrophe. Into the 90’s, the incidence of human plague is on the rise. Currently, the epidemic area of the disease is still judged to be passive hemagglutination test and other enzyme-linked immunosorbent assay (ELISA) based on routine examination (microscopy, culture, phage lysis and animal experiments), but these methods There are still some issues in terms of reliability and simplicity. Therefore, the establishment of highly sensitive and rapid detection of Yersinia pestis method is still urgent and necessary. In order to make more reliable use of nucleic acid technology, especially in cases where it is difficult to isolate pathogens, direct plague outbreaks in foci