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本文介绍人肝癌细胞株细胞及其移植瘤组织经冻存、复苏后检测琥珀酸脱氢酶(SDE)活性、成瘤率和电镜检查。选择10％DMSO或10％甘油作为冷冻保护剂来冻存细胞。它们先在冰箱里缓慢降温冷却（-0．8℃／min），后在液氮罐口的液氮气中冷冻（-10℃／min）1天。最后置入液氮内。冷冻保护后的冻存或组织复苏后显示高的SDE活性，10％DMSO冻存的细胞SDE活性比10％甘油者高，两者相比有显著差别（P＜0．01）。尽管两组复苏后的接种成瘤率相同（80％），但它们移植25天后，前者移植瘤瘤重比后者重1．66倍。电镜下，两种冻存液冻存的细胞都有“暗”和“明”细胞，它们都有完整的线粒体膜和质膜。推荐10％DMSO作为移植瘤冷冻保护液。
This article describes the human hepatocellular carcinoma cell line and its transplanted tumor tissue after cryopreservation, recovery after the detection of succinate dehydrogenase (SDE) activity, tumor formation rate and electron microscopy. Choose 10% DMSO or 10% glycerol as a cryoprotectant to freeze the cells. They were cooled slowly in the refrigerator (-0.8°C/min) and then frozen in liquid nitrogen at -10°C/min for 1 day. Finally placed in liquid nitrogen. After cryopreservation, cryopreservation or tissue resuscitation showed high SDE activity, and 10% DMSO cryopreserved cells had higher SDE activity than 10% glycerol. There was a significant difference between the two (P<0.01). Although the rate of tumorigenesis after resuscitation was the same in both groups (80%), the transplanted tumors weighed 1.66 times more than the latter after 25 days of transplantation. Under the electron microscope, cells that were frozen in both cryovials have “dark” and “bright” cells, and they all have intact mitochondrial and plasma membranes. 10% DMSO is recommended as a cryoprotectant for transplanted tumors.