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目的研究17-β-雌二醇对人胃癌细胞增殖周期的影响,探讨胃肠道肿瘤的内分泌治疗的可能性。方法采用人胃癌SGC-7901细胞株,免疫组化染色证实其雌激素受体为阳性,在细胞培养期间,分别给予雌二醇10~(-7)mol/L及雌二醇加三苯氧胺1mcg/ml处理12 h,流式细胞仪检测各期细胞数目的相对比例和增殖指数。结果细胞增殖指数在雌二醇组、对照组和三苯氧胺处理组分别为0.5705,0.4787和0.4830。当用雌二醇处理后其S期细胞数目明显增加。结果显示雌二醇可刺激该细胞的生长,而三苯氧胺可抑制这种作用。结论雌二醇对雌激素受体阳性的胃癌细胞有增殖营养效应,这种作用可为雌激素受体抑制剂三苯氧胺所阻断,可用来控制胃癌的生长。
Objective To study the effect of 17-β-estradiol on the proliferation cycle of human gastric cancer cells and explore the possibility of endocrine therapy of gastrointestinal tumors. Methods Human gastric cancer SGC-7901 cell line was used. Immunohistochemical staining confirmed its positive estrogen receptor. During cell culture, estradiol 10~(-7) mol/L and estradiol plus tamoxifen 1mcg/ were given during cell culture. After being treated with ml for 12 h, the relative proportion and cell proliferation index of the cells in each phase were measured by flow cytometry. Results The cell proliferation index was 0.5705, 0.4787 and 0.4830 in the estradiol group, the control group, and the tamoxifen treatment group, respectively. When treated with estradiol, the number of S-phase cells significantly increased. The results showed that estradiol stimulated the growth of the cells, and tamoxifen inhibited this effect. Conclusion Estradiol has a proliferative and nutritional effect on estrogen receptor-positive gastric cancer cells. This effect can be blocked by the estrogen receptor inhibitor tamoxifen and can be used to control the growth of gastric cancer.