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目的:探讨缺氧诱导因子1α(HIF-1α)在胆囊癌组织中的表达及其与临床病理因素相关性和对胆囊癌患者胆囊癌侵袭转移中的影响。方法:选择2009年1月—2010年12月收治的胆囊癌患者80例以及75例胆囊炎的患者,应用免疫组织化学法检测胆囊癌组织标本及慢性胆囊炎胆囊黏膜组织标本中HIF-1α表达情况。结果:HIF-1α在胆囊癌组织中表达强,胞核及胞浆着色,阳性表达(A值)为163.44±25.46;慢性胆囊炎胆囊黏膜上皮HIF-1α表达较弱,胞核及胞浆着色,阳性表达(A值)为75.35±15.43。两者比较差异有显著统计学意义(P<0.05)。在胆囊癌TNM不同分期患者中,Ⅰ期和Ⅱ期者HIF-1α表达明显低于Ⅲ期和Ⅳ期者的HIF-1α表达,差异有统计学意义(P<0.05);在胆囊癌无淋巴结转移组HIF-1α表达明显低于淋巴结转移组中HIF-1α,差异有统计学意义(P<0.05)。结论:HIF-1α表达水平是影响胆囊癌患者侵袭转移中的独立影响因素。
Objective: To investigate the expression of hypoxia-inducible factor 1α (HIF-1α) in gallbladder carcinoma and its relationship with clinicopathological factors and the influence on the invasion and metastasis of gallbladder carcinoma in patients with gallbladder cancer. Methods: 80 cases of gallbladder carcinoma and 75 cases of cholecystitis admitted to our hospital from January 2009 to December 2010 were enrolled in this study. The expression of HIF-1α in gallbladder carcinoma specimens and gallbladder mucosa specimens were detected by immunohistochemistry Happening. Results: The expression of HIF-1α in gallbladder cancer tissues was strong, the nucleus and cytoplasm were stained, the positive expression (A value) was 163.44 ± 25.46. The expression of HIF-1α in gallbladder mucosal epithelium of chronic cholecystitis was weak and the staining of nucleus and cytoplasm , The positive expression (A value) was 75.35 ± 15.43. The difference was statistically significant (P <0.05). In patients with TNM at different stages of gallbladder cancer, the expression of HIF-1α in stage Ⅰ and stage Ⅱ was significantly lower than that in stage Ⅲ and stage Ⅳ, the difference was statistically significant (P <0.05); in gallbladder cancer without lymph node The expression of HIF-1α in metastasis group was significantly lower than that in lymph node metastasis group, the difference was statistically significant (P <0.05). Conclusion: The expression of HIF-1α is an independent influencing factor in invasion and metastasis of gallbladder carcinoma.