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本文以 BAIB/C艾氏腹水癌荷瘤小鼠为模型,用[~3H]-甲基掺入法测定了各增殖时相的艾氏腹水癌细胞内DNA甲基化酶活力水平,DNA甲基化作用是生物体内普遍存在的一种基因修饰作用,这种修饰作用发生在DNA复制之后,转录之前,其过程为在DNA甲基化酶作用下,以S-腺苷酞-甲硫氨酸为甲基供体,使DNA特异序列上的胞嘧啶(5mc)甲基化.由于甲基的移位,故影响基因的表达,DNAA甲基化作用在基因表达与调控中起重要作用,DNA甲基化酶活力大小可反应出DNA甲其化水平的高低.是从基因表达与调控角度阐明抗肿瘤药物作用分子机理的重要手段之一.
In this study, BALB/i Ehrlich ascites carcinoma-bearing mice were used as models, and DNA methylation activity levels in Ehrlich ascites carcinoma cells at each proliferation phase were determined by [~3H]-methyl incorporation method. The basification is a kind of gene modification commonly found in organisms. This modification takes place after DNA replication, before transcription. The process is under the action of DNA methylase, S-adenosine-methylthio ammonia. The acid is a methyl donor, which methylates cytosine (5mc) on the DNA-specific sequence. As the translocation of methyl, it affects gene expression, and DNAA methylation plays an important role in gene expression and regulation. DNA methylation activity can reflect the level of DNA hypermethylation. It is one of the important ways to elucidate the molecular mechanism of antitumor drugs from the perspective of gene expression and regulation.