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目的探讨苦参碱体内对小鼠精子活性、精子密度、畸形发生率,以及对雌性小鼠受孕情况的影响。方法50只NIH雄性小鼠随机分成5组,分别为空白对照组、供试品苦参碱设低、中、高3个剂量组,分别灌胃给予2、4、8mg.kg-1和醋酸棉酚20 mg.kg-1阳性对照组。连续灌胃给药21d后,各组雄性小鼠分别与10只NIH雌性小鼠合笼5d后再分笼(合笼及分开饲养期间雄性小鼠持续给药),分笼5 d后脱颈椎处死各组雄性小鼠,观察并计算精子活性、精子密度及畸形发生率。分笼后第十天解剖、观察并计数雌鼠怀孕胎数。结果苦参碱给药组及阳性对照组精子活性与空白对照组相比较均有显著差异(P<0.05),畸形发生率有显著差异(P<0.01),各给药组、阳性对照组在短时间内(31d)与空白对照组相比,精子密度和雌性小鼠受孕胎数均未产生显著的影响(P>0.05).结论苦参碱口服给药后对小鼠精子活性有一定抑制作用且具有明显的致畸作用。
Objective To investigate the effects of matrine on the sperm motility, sperm density and the incidence of malformation in mice and the effect of matrine on female mice. METHODS: Fifty NIH male mice were randomly divided into 5 groups: blank control group, low-, middle-, and high-dose groups of matrine, which were given 2, 4, 8 mg.kg-1 and acetic acid, respectively. Gossypol 20 mg.kg-1 positive control group. After continuous intragastric administration for 21 days, each group of male mice and 10 NIH female mice were caged for 5 days and then divided into cages (the male mice were continuously administered during caged and separated feeding), and the cervical vertebra was dissected after 5 days of cage separation. Male mice of each group were sacrificed, and sperm activity, sperm density, and incidence of malformation were observed and calculated. On the tenth day after the cage was divided, the numbers of female pregnant fetuses were observed and counted. Results Compared with the blank control group, the sperm activity in the matrine-treated group and the positive control group was significantly different (P<0.05), and the incidence of malformation was significantly different (P<0.01). Each administration group and positive control group were Within a short time (31d) compared with the control group, the sperm density and the number of fetuses in the female mice did not have a significant effect (P>0.05). Conclusions Matrine has certain inhibition of sperm motility after oral administration. The role and has a significant teratogenic effect.